Do you have a protocol or advised antibodies to carry out ICC staining on my completely differentiated ALI cultures?
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Cells from the Immune System The immune system is comprised of numerous subsets of cells that work with each other to shield the host from infections and malignancies.
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This product or service is made for use in the subsequent analysis area(s) as Component of the highlighted workflow stage(s). Investigate these workflows to learn more regarding the other products we offer to support each exploration region. Exploration Region Workflow Levels
The readings may also be conducted before the culture is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
EasySep™ is a fast and easy immunomagnetic cell separation engineering that lets you isolate virtually any cell sorts from several different sample resources in as minimal as eight minutes. This online video describes the EasySep™ technology.
Note: Move forward to antibody staining if undertaking Are living cell analysis right after this phase. Alternatively, progress to fixable dye staining and PFA fixation.
In case cell clumping is noticed after thawing cryopreserved cells, it is suggested to filter aggregated suspensions by way of a 37 µm cell strainer (e.g. Catalog #27250) for exceptional final results. In such a case, the run time to the CellPore™ 지방흡입 Transfection System might must be increased to 10 seconds.
To assess the regional specificity in the tiny vs huge airway, you'll be able to carry out the following assays:
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